ABSTRACT
Background: Insulin-like growth factor 1 has an important role in cell proliferation and growth. Polymorphism in IGF-1 gene changes the level of this hormone and increases the risk of colorectal cancer. The aim of this study is to assess the incidence of IGF-1 polymorphism rs5742612 in Iranian population and to investigate the influence of this polymorphism in increasing the risk of colorectal cancer
Methods and Materials: Genotyping of IGF-1 gene was performed in a series of 110 colorectal cancer patients and 110 controls by using polymerase chain reaction and restriction fragment length polymorphism genotyping [PCR-RFLP] assays. We calculated odds ratio and confidence interval [CI] of IGF-1 genotypes to determine if these polymorphisms are associated with colorectal cancer
Results: No significant association was found between polymorphism of IGF-1 gene [rs5742612] and increased risk of colorectal cancer [p=0.92]. The odds ratio for the heterozygous genotype CT versus the normal genotype CC was 1.3 [95% CI:0.3-6.27] and the odds ratio for the mutant genotype TT versus the normal genotype CC was 0.0. The frequency of the mutant allele was 2.5%
Conclusion: These findings suggest that IGF-1 rs5742612 polymorphism is not associated with increased risk of colorectal cancer